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Pretreatment times and dose ranges for the drug discrimination assay were selected based on the time of peak depression in the locomotor activity assay in mice.
However, because of their subjective properties, it is necessary to set up a more objective automated measurement to determine their neurotoxicity.
Discriminative stimulus effects were tested in rats trained to discriminate Δ9-tetrahydrocannabinol (3 mg/kg, 30-min pretreatment).
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Drug was administered 5 times every other day, and the first trial was performed 2 h after the last administration.
As mentioned previously, short-onset compounds have a greater abuse liability; further, compounds that have fewer adverse effects while they are active are likely to be preferred.
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