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Twenty-four male Sprague-Dawley rats were obtained from Envigo (Houston, TX).
The FOB test was performed using published procedures (Moser et al., 1989) with some modifications.
Elegans has limitations such as a low abundance of ester hydrolysis metabolite, in general, the metabolic profiles were in good agreement.
Smoke of e-cigarettes contains toxic metals and nicotine, and due to the added flavours it has even become more easy to become addicted to vaping.
Substantial depressant effects were observed within the first 10 min, and maximal depression was observed between 10–40 min and lasted up to 2.5 to 3 h at the highest dose tested (0.5 mg/kg).
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