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In locomotor activity, methamphetamine treated group showed approximately 4.2 times increase compared to the negative control treated group.
It should be noted that % peak area abundance ratios do not necessarily reflect absolute concentrations due to differences in ionization capacity and matrix effects bias for each metabolite.
Figure 1 shows average horizontal activity counts/10 min as a function of time (0–4 h) and dose of Δ9-THC.
The chemical structures of the recent synthetic cannabinoids are unlike that of Δ9-THC, but are largely based on the structure of older synthetic cannabinoids that are known to have substantial abuse liability (Fig. 1).
Response-rate data were analyzed by one-way repeated-measure analysis of variance.
AMB-FUBINACA has been implicated in severe adverse effects in recreational users (Adams et al., 2017; Hamilton et al., 2017), which suggests that the range between behaviorally active and toxic doses of AMB-FUBINACA is narrow.
Most of the abnormalities were normalized in synthetic cannabinoid treated mice although those abnormal behaviors remained in methamphetamine treated animals after 2 hr of administration.
Effects of individual doses were compared to the vehicle control value using a priori contrasts.
Subsequent testing identified 5F-ADB to have been present in a total of ten people who had died from unexplained drug overdoses in Japan between September 2014 and December 2014.
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