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Forensic autopsy of both victims was performed four days after the time of death following the Recommendation No.R (99)3 of the Council of Europe on medico-legal autopsies.
The product ions at m/z 316 and 231 in B17 were 14 Da higher than that of product ions 302 and 217, respectively, in B18, consistent with the formation of a butanoic acid metabolite.
The reaction was quenched by adding ice-cold ACN (1 mL) to the mixture, vortex mixed and then centrifuged at 13,000 g for 10 min.
All of the compounds tested in the present study depressed locomotor activity as is typical for other synthetic cannabinoids (see review by Wiley et al., 2017).
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