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Drug was administered 5 times every other day, and the first trial was performed 2 h after the last administration.
Oxidation of 4′-hydroxybutyl moiety in B2 led to formation of 4′-carboxybutyl metabolite (B4) having a precursor ion of m/z 362, which was 14 Da higher than the m/z for B2 (loss of two hydrogen atoms with addition of a carbonyl group).
Tremors were observed in mice 30 minutes following 1 mg/kg AMB-FUBINACA in the present study.
Co-use of pregabalin (oral) and crack cocaine (smoked) were also reported.
Similar to the in-vivo findings, 4F-MDMB-BINACA ester hydrolysis (B22) was the major metabolite for both HepG2 and HLM models, consistent with the known hydrolytic activity of CES reported .
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